goat anti ki67 antibody  (R&D Systems)

Journal: iScience

Article Title: Human OPN-derived peptide SV prevents BPD model through interacting with KIF5B to repair mitochondrial damage and inhibit apoptosis

doi: 10.1016/j.isci.2025.113691

Figure Lengend Snippet: SV improves the LPS-induced BPD animal model (A) Body weight changes from 1 to 7 days in control, LPS, and LPS+SV groups of mice, N = 6. (B) Lung morphology of control, LPS, and LPS+SV groups, N = 6. (C) Lung histopathologic changes (H&E), N = 6. Mean linear intercept (MLI) quantitative represents the mean airspace diameter and mean alveolar number (MAN) quantitative represents the mean number of alveoli. Scale bars: 500 μm/100 μm. (D) Representative images of TUNEL immunostaining showing the apoptosis (green staining) in rat lung tissue of control, LPS and LPS+SV groups, N = 6. Representative images of Ki67 immunostaining showing the proliferation (red staining) in rat lung tissue of control, LPS, and LPS+SV groups; N = 6. Scale bars: 500 μm. (E) qPCR analysis showed the mRNA levels of Bcl-2, Bax, SPC, IL-1β, and IL-6, N = 6. (F and G) Western blot showed the protein levels of Bcl-2, Bax, SPC, and VEGFA, N = 6. Unpaired t test, ∗/#/ p < 0.05,∗∗/##/ p < 0.01, and ∗∗∗/###/ p < 0.001. CTL, control; LPS, BPD induced by lipopolysaccharide. The LPS concentration was 500 μg/kg and the SV intervention concentration was 200 μg/kg depending on the weight of the pups per day.

Article Snippet: The samples were subsequently incubated with primary antibodies, including goat anti-mouse Ki67 (CST, 1:100), and then subjected to TUNEL staining at RT for 1h.

Techniques: Animal Model, Control, TUNEL Assay, Immunostaining, Staining, Western Blot, Concentration Assay

Journal: iScience

Article Title: Human OPN-derived peptide SV prevents BPD model through interacting with KIF5B to repair mitochondrial damage and inhibit apoptosis

doi: 10.1016/j.isci.2025.113691

Figure Lengend Snippet: SV improves the hyperoxia-induced BPD animal model (A) Body weight changes from 1 to 7 days in control, Hyp, and Hyp+SV groups of mice, N = 6. (B) Lung morphology of control, Hyp, and Hyp+SV groups of mice, N = 6. (C) Lung histopathologic changes (H&E), N = 6. MLI quantitative represents the mean airspace diameter and MAN quantitative represents the mean number of alveoli. Scale bars: 500 μm/100 μm. (D) Representative images of TUNEL immunostaining showing the apoptosis (green staining) in rat lung tissue of control, Hyp, and Hyp+SV groups. Representative images of Ki67 immunostaining showing the proliferation (red staining) in rat lung tissue of control, LPS, and LPS+SV groups, N = 6. Scale bars: 500 μm. (E) qPCR analysis showed the mRNA levels of Bcl-2, Bax, SPC, IL-1β, and IL-6, N = 6. (F and G) Western blot showed the protein levels of Bcl-2, Bax, SPC, and VEGFA, N = 6. Unpaired t test, ∗/#/ p < 0.05,∗∗/##/ p < 0.01, and ∗∗∗/###/ p < 0.001. CTL, control; Hyp, BPD induced by hyperoxia. The SV intervention concentration was 200 μg/kg depending on the weight of the pups per day.

Article Snippet: The samples were subsequently incubated with primary antibodies, including goat anti-mouse Ki67 (CST, 1:100), and then subjected to TUNEL staining at RT for 1h.

Techniques: Animal Model, Control, TUNEL Assay, Immunostaining, Staining, Western Blot, Concentration Assay